BIO-HELIX - PM006-0500
BlueRAY Prestained Protein Ladder（10 to 180 kDa）
Size: 500 μl
The BlueRAY Prestained Protein Ladder is a combination of 10 pre-stained proteins with molecular weights from 11 to 180 kDa. The 10 recombinant proteins are covalently coupled with blue chromophore, while 1 green band at 25 kDa and a red band at 75 kDa, serve as reference bands. The BlueRAY Prestained Protein Ladder keeps track of the size and separation of proteins during SDS-polyacrylamide gel electrophoresis, approximating protein size, and validating Western transfer efficiency on PVDF, nylon, or nitrocellulose membranes.
-Guide for Molecular Weight Estimation (kDa)
Migration patterns of BlueRAY Prestained Protein Ladder in different electrophoresis conditions are listed below :
3 μl or 5 μl per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively. 2.5 μl per well for general Western transferring.
Note: The molecular weight of each protein (kDa) was measured against an unstained protein ladder in every electrophoresis condition. Additional data should be considered for a more accurate adjustment.
Storage Buﬀer :
Approximately 0.2~0.4 mg/ml of each protein in buffer (20 mM Trisphosphate pH 7.5 at 25°C), 2% SDS, 0.2 mM Dithiothreitol, 3.6 M Urea, and 15% (v/v) Glycerol.
Quality Control :
5 μl of BlueRAY Prestained Protein Ladder (PM006-0500) resolves 10 bands in 4-20% SDS-PAGE (Tris-glycine buffer) and after Western blotting to PVDF membrane.
Stable for up to 2 weeks at 25°C.
Stable for up to 3 months at 4°C.
Stable for up to 24 months at -20°C.
Lane 1: PM019-0500 (Bio-Helix) 5ul
Lane 2: PM008-0500 (Bio-Helix) 5ul
Lane 3: PM007-0500 (Bio-Helix) 5ul
Lane 4: PM006-0500 (Bio-Helix) 5ul
Lane 5: PM001-0500 (Bio-Helix) 5ul
Lane 6: PM019-0500 (Bio-Helix) 5ul
Lane 7: PM001-0500 (Bio-Helix) 5ul
Lane 8: PMU12-0500 (Bio-Helix) 5ul
Lane 9: thermo Mark12 5ul
Lane 10: Bio-Rad 161-0317 5ul
*NuPAGE Bis-Tris 4-12% was used to run SDS-PAGE in MOPS and MES buffer. 5ul of protein ladder samples was loaded into each lane
- Vertical Electrophoresis system
- Power supplies
- Vortex or equivalent