BIO-HELIX - PM009-0500
IRIS9 Prestained Protein Ladder（15 to 180 kDa）
Size: 500 μl
The IRIS9 Prestained Protein Ladder is a combination of 9 pre-stained proteins with molecular weights from 15 to 180 kDa. The 9 recombinant proteins are covalently coupled with blue chromophore, while 2 red bands at 70 kDa and 30 kDa, a green band at 15 kDa and a newly designed peacock green band at 60 kDa serve as reference bands. The IRIS9 Prestained Protein Ladder keeps track of the size and separation of proteins during SDS-polyacrylamide gel electrophoresis, approximating the target protein size and validating the Western transfer efficiency on PVDF, nylon, or nitrocellulose membranes.
Guide for Molecular Weight Estimation (kDa)
Refer to the IRIS9 Prestained Protein Ladder patterns in various electrophoresis conditions:
3 μl or 5 μl per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively. 2.5 μl per well for general Western transferring.
Note: The molecular weight of each protein (kDa) was measured against an unstained protein ladder in every electrophoresis condition. Additional data should be considered for a more accurate adjustment.
Storage Buﬀer :
0.2~0.4 mg/ml of each band of protein is stored in a mixture of solution that contains: 20 mM Trisphosphate at pH 7.5, 2% SDS, 0.2 mM Dithiothreitol, 3.6 M Urea, and 15% (v/v) Glycerol.
Quality Control :
5μl of IRIS9 Prestained Protein Ladder (PM009) resolves 9 bands in 4-20% SDS-PAGE (Tris-glycine buffer) and after Western blotting to PVDF membrane.
Stable for up to 2 weeks at 25°C.
Stable for up to 3 months at 4°C.
Stable for up to 24 months at -20°C.
Figure 1. Gel image of SDS-PAGE. 5 ul of prestained protein ladder sample is loaded into each lane.
Lane A, IRIS9 Prestained Protein Ladder (PM009 Iris9), storage at -20°C for 14 days.
Lane B, IRIS9 Prestained Protein Ladder (PM009 Iris9), storage at 4°C for 14 days.
Lane C, IRIS9 Prestained Protein Ladder (PM009 Iris9), storage at 37°C for 14 days.
- Vertical Electrophoresis system
- Power supplies
- Vortex or equivalent