BIO-HELIX - PS002-L05X

5X Concentrate COOMASSIE nano Protein Staining Solution

Size: 1 L



Note: Concentrate / Protein Stain / Instant Stain / Coomassie Blue / Non-toxic / SDS-PAGE / Gel Electrophoresis / Polyacrylamide Gel /
Categories: Staining Reagent

5X Concentrate COOMASSIE nano ╴Innovatively developed Next-Generation Protein Stain for faster and environment-friendly gel visualization.

5X COOMASSIE nano, innovatively enhanced by the proprietary nano-technology, is a concentrated, easy-to-reconstitute protein staining solution for SDS-PAGE gels and is also compatible with mass spectrophotometry. Its next generation formula offers faster protein detection and higher sensitivity while eliminating the conventional need for destaining and wash. In the absence of hazardous substances, such as methanol and acetic acid, 5X COOMASSIE nano is considered to be safe and environmentally friendly by reducing carbon footprint by as much as 80%.

 

Figure I. The same performance between 1X & 5X COOMASSIE nano 

We used ImageJ to analysis the 85kDa band of lane 2 (red arrow), comparing the band intensity between 1X and 5X Coomassie nano. The variation is under 10%.

Lane 1:Prestain Protein Ladder #PM019-0500
Lane 2-15:2X Dilutions of Unstain Ladder #PMU12-0500

Figure II : 5X COOMASSIE nano stability test at 37℃

We used ImageJ to analysis the 85kDa band of lane 5 (red arrow), comparing the band intensity between different storage time at 37℃. The variation is small 10%.

 

Lane 1:Prestain Protein Ladder #PM019-0500
Lane 2-15:2X Dilutions of Unstain Ladder #PMU12-0500

※ Comparison data:

  1. Prepare a 5L bottle
  2. Unpack PS002-L05X and pour the liquid content into the bottle.
  3. Rinse residual reagent in the original bottle with deionized water.
  4. Add deionized water until the volume reaches 5L.

 

  1. Make 5X Concentrate COOMASSIE nano into 1X COOMASSIEnano protein staining solution.
  2. Carefully mix COOMASSIE nano up and down few times before use.
  3. After SDS-PAGE has finished, remove the gel from the cassette and submerge it in the proper amount of COOMASSIE nano stain, enough to cover the gel. Lightly agitate the staining box for 10 to 30 minutes at room temperature.
  4. As there is no need for washing or destaining, please continue to carefully remove the staining solution and rinse the excess stain in the gel with water.
  5. Image the gel.
  1. There is no risk of excessive staining. Instead, superior visibility for each band is achieved when the gel is submerged overnight.
  2. Carefully mix COOMASSIE nano up and down few times before use.
  3. If the gel is thicker than mini-gel (1mm), it will take longer to incubate.
  4. The staining solution may contain blue clumps. This is normal and easily dissolved in distilled water