BIO-HELIX - DP001-0100

NanoTaq Hot-Start DNA Polymerase

Size: 100rxns (500units /100ul)



Note: Hot-Start Taq / Universal Annealing Protocol / Thermostability / COVID-19 (SARS-CoV-2) / qPCR / real-time PCR
Categories:

Bio-Helix NanoTaq DNA Polymerase was designed as one of enhanced hot start enzyme DNA Polymerases which provide the convenience and reliability toward your research destination. NanoTaq was engineered with nano technology complex, which is an innovative creation, different from the traditional methods of hot start enzymes made. The proven features of NanoTaq covers reactions at room temperature using the same protocol and cycling conditions as conventional Taq DNA polymerases, reducing nonspecific primer annealing, improving product yield and using for PCR products up to 5kb.


Comparison of commercially Hot-Start DNA Polymerase.

Figure 1. Comparison of commercially Hot-Start DNA Polymerase. 406 bp, 653 bp, and 320 bp fragments were amplified from 3.6 ng of human genomic DNA in 20 μL reactions using (A) NanoTaq Hot-Start DNA Polymerase, (B) DreamTaq™ Hot Start DNA Polymerase, (C) KAPA2G Fast HotStart PCR Kit. The programs were according to different Hot-Start DNA Polymerase suggested. The size marker is Bio-Helix 1Kb DNA Ladder RTU.

 

Efficient amplification of DNA sequences with a range of GC content.

Figure 2. Efficient amplification of DNA sequences with a range of GC content. A series of DNA fragments of increasing GC content were amplified from human gDNA. NanoTaq Hot-Start DNA Polymerase was used for targets with >50% GC.

► Reactions at room temperature using the same protocol and cycling conditions as conventional Taq DNA polymerases
► Reduce nonspecific primer annealing
► Improve product yield
► Use for PCR products up to 5~6kb

NanoTaq DNA polymerase provides both specificity and yield.

NanoTaq DNA polymerase provides both specificity and yield

A

NanoTaq

B

Thermo DreamTaq

C

Thermo DreamTaq HS

D

Roche KAPA2G HS

E

Invitrogen Platinum HS

 

 

Comparison of non-specific amplifying effects to major suppliers. All amplifications were performed in accordance with manufacturer’s instructions to amplify 320 to 941 bp amplicons from human genomic DNA. NanoTaq provided both higher specificity and yield products with least band shifting compared to other commercial antibody-mediated hot start polymerase.