BIO-HELIX - PM008-0500

BLUelf Prestained Protein Ladder(5 to 245 kDa)

Size: 500 μl



Note: Protein Marker / Protein Standard / SDS-PAGE / Western Blot
Categories: Protein Marker/Ladder

The BLUelf Prestained Protein Ladder is a combination of 13 pre-stained proteins with molecular weights from 5 to 245 kDa. The 13 recombinant proteins are covalently coupled with blue chromophore, while 1 green band at 25 kDa and a red band at 75 kDa serve as reference bands. The BLUelf Prestained Protein Ladder keeps track of the size and separation of proteins during SDS-polyacrylamide gel electrophoresis, approximating protein size and validating Western transfer efficiency on PVDF, nylon, or nitrocellulose membranes.

 

Guide for Molecular Weight Estimation (kDa)
Refer to the BLUelf Prestained Protein Ladder patterns in various electrophoresis conditions:


 

3 μl or 5 μl per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively. 2.5 μl per well for general Western transferring.

Note: The molecular weight of each protein (kDa) was measured against an unstained protein ladder in every electrophoresis condition. Additional data should be considered for a more accurate adjustment.

 

Storage Buffer : 
Approximately 0.1~0.4 mg/ml of each protein in the buffer (20 mM Tris-phosphate, pH 7.5 at 25°C), 2 % SDS, 0.2 mM Dithiothreitol, 3.6 M Urea, and 15 % (v/v) Glycerol.

Quality Control :
5 μl of BLUelf Prestained Protein Ladder (PM008-0500) resolves 13 bands in 4-15% SDS-PAGE (Tris-glycine buffer) and after Western blotting to a nitrocellulose membrane.

Storage :
Stable for up to 2 weeks at 25°.
Stable for up to 3 months at 4°C.
Stable for up to 24 months at -20°C.

Note :
The apparent molecular weight (kDa) of each protein has been determined by calibration against unstained protein standards; supplemental data should be considered for more accurate adjustment in different electrophoresis conditions. All products are for research use only.

Caution :
Not intended for human, animal diagnostic or therapeutic uses.

 

Lane 1: PM019-0500 (Bio-Helix) 5ul
Lane 2: PM008-0500 (Bio-Helix) 5ul
Lane 3: PM007-0500 (Bio-Helix) 5ul
Lane 4: PM006-0500 (Bio-Helix) 5ul
Lane 5: PM001-0500 (Bio-Helix) 5ul
Lane 6: PM019-0500 (Bio-Helix) 5ul
Lane 7: PM001-0500 (Bio-Helix) 5ul
Lane 8: PMU12-0500 (Bio-Helix) 5ul
Lane 9: thermo Mark12 5ul
Lane 10: Bio-Rad 161-0317 5ul

*NuPAGE Bis-Tris 4-12% was used to run SDS-PAGE in MOPS and MES buffer. 5ul of protein ladder samples were loaded into each lane.


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