GeneDireX - PM008-0500

BLUelf Prestained Protein Ladder

Size: 500 μl



Note: GeneDireX / Protein Marker / Protein Standard / SDS-PAGE / Western Blot
Categories: Protein Marker/Ladder

The BLUelf Prestained Protein Ladder is a three-color protein standard with 13 pre-stained proteins covering a wide range molecular weights from 3.5 to 245 kDa. Proteins are covalently coupled with a blue chromophore except for two reference bands (one green and one red band at 25 kDa and 75 kDa respectively) when separated on SDS-PAGE (Tris-glycine buffer). The BLUelf Prestained Protein Ladder is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins. The ladder is supplied in gel loading buffer and is ready to use. Do not heat, dilute, add reducing agent before loading.

Guide for Molecular Weight Estimation (kDa)
Migration patterns of BLUelf Prestained Protein Ladder in different electrophoresis conditions are listed below :


 

• 3 μl or 5 μl per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively.
• 1.5~2.5 μl per well for general Western transferring.
• Apply more for thicker (> 1.5 mm) or larger gel.

 

Contents :
Approximately 0.1~0.4 mg/ml of each protein in the buffer (20 mM Tris-phosphate, pH 7.5 at 25°C), 2 % SDS, 0.2 mM Dithiothreitol, 3.6 M Urea, and 15 % (v/v) Glycerol.

Quality Control :
Under suggested conditions, BLUelf Prestained Protein Ladder resolves 13 major bands in polyacrylamide gel with appropriate buffers and after Western blotting to a nitrocellulose membrane.

Storage :
Stable for up to 3 months at 4°C
Stable for up to 2 weeks at 25°Clong term storage, store at -20°C

Note :
The apparent molecular weight (kDa) of each protein has been determined by calibration against unstained protein standards; supplemental data should be considered for more accurate adjustment in different electrophoresis conditions. All products are for research use only.

Caution :
Not intended for human or animal diagnostic or therapeutic uses.

 

Lane 1: GeneDireX PM019-0500 (Bio-Helix) 5ul
Lane 2: GeneDireX PM008-0500 (Bio-Helix) 5ul
Lane 3: GeneDireX PM007-0500 (Bio-Helix) 5ul
Lane 4: GeneDireX PM006-0500 (Bio-Helix) 5ul
Lane 5: GeneDireX PM001-0500 (Bio-Helix) 5ul
Lane 6: GeneDireX PM019-0500 (Bio-Helix) 5ul
Lane 7: GeneDireX PM001-0500 (Bio-Helix) 5ul
Lane 8: GeneDireX PMU12-0500 (Bio-Helix) 5ul
Lane 9: thermo Mark12 5ul
Lane 10: Bio-Rad 161-0317 5ul

*NuPAGE Bis-Tris 4-12% was used to run SDS-PAGE in MOPS and MES buffer. 5ul of protein ladder samples was loaded into each lane.

Name Download
GeneDireX_PM008-0500_Protocol GeneDireX_PM008-0500_Protocol.pdf
GeneDireX_PM008-0500_SDS GeneDireX_PM008-0500_SDS.pdf

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