GeneDireX - PM019-0500
BlueAQUA Prestained Protein Ladder
Size: 500 μl
The BlueAQUA Prestained Protein Ladder is a blue protein standard with 11 pre-stained proteins covering a wide range molecular weights from 10 to180 kDa. Proteins are covalently coupled with a blue chromophore, and two reference bands (at 25 kDa and 72 kDa respectively) are enhanced in intensity when separated on SDS-PAGE (Tris-glycine buffer).
The BlueAQUA Prestained Protein Ladder is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins.
The ladder is supplied in gel loading buffer and is ready to use. Do not heat, dilute, add reducing agent before loading.
Guide for Molecular Weight Estimation (kDa)
Migration patterns of BlueAQUA Prestained Protein Ladder in different electrophoresis conditions are listed below :
• 3 μl or 5 μl per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively.
• 1.5~2.5 μl per well for general Western transferring.
• Apply more for thicker (> 1.5 mm) or larger gel.
Approximately 0.1~0.5 mg/ml of each protein in the buffer (20 mM Tris-phosphate, pH 7.5 at 25°C), 2 % SDS, 0.2 mM Dithiothreitol, 3.6 M Urea, and 15 % (v/v) Glycerol).
Quality Control :
Under suggested conditions, BlueAQUA Prestained Protein Ladder resolves 11 major bands in 15% SDS-PAGE (Tris-glycine buffer) and after Western blotting to a nitrocellulose membrane.
Stable for up to 3 months at 4°C
Stable for up to 2 weeks at 25°C
For long-term storage, store at -20°C
Lane 1: GeneDireX PM019-0500 (Bio-Helix) 5ul
Lane 2: GeneDireX PM008-0500 (Bio-Helix) 5ul
Lane 3: GeneDireX PM007-0500 (Bio-Helix) 5ul
Lane 4: GeneDireX PM006-0500 (Bio-Helix) 5ul
Lane 5: GeneDireX PM001-0500 (Bio-Helix) 5ul
Lane 6: GeneDireX PM019-0500 (Bio-Helix) 5ul
Lane 7: GeneDireX PM001-0500 (Bio-Helix) 5ul
Lane 8: GeneDireX PMU12-0500 (Bio-Helix) 5ul
Lane 9: thermo Mark12 5ul
Lane 10: Bio-Rad 161-0317 5ul
*NuPAGE Bis-Tris 4-12% was used to run SDS-PAGE in MOPS and MES buffer. 5ul of protein ladder samples was loaded into each lane.