The PMF 11 Fluorescent Prestained Protein Ladder is a combination of 11 pre-stained proteins with molecular weights from 11 kDa to 300 kDa. The 11 recombinant proteins are stained with blue, red, and green dyes and also provide for fluorescent visualization, with 3 red bands at 27kDa, 80 kDa, and 300 kDa as reference bands. For fluorescent visualization, 10 stained proteins can be detected using the 440 nm – 460 nm excitation wavelength, and emission wavelengths at 700 nm – 740 nm. Primarily, the FMF 11 Fluorescent Prestained Protein Ladder keeps track of the size and separation of proteins during SDS-polyacrylamide gel electrophoresis, approximating the target protein size and validating the Western transfer efficiency on PVDF, nylon, or nitrocellulose membranes.

Storage Buffer :

0.2~0.4 mg/ml of each band of protein is stored in a mixture of solution that contains: 20 mM Trisphosphate at pH 7.5, 2% SDS, 0.2 mM Dithiothreitol, 3.6 M Urea, and 15% (v/v) Glycerol.

Quality Control :
5 μl of PMF 11 Fluorescent Protein Ladder (PMF11) resolves 11 bands in 4-20% SDS-PAGE (Tris-glycine buffer) and after Western blotting to PVDF membrane.

Storage :
Stable for up to 2 weeks at 25°C.
Stable for up to 3 months at 4°C.
Stable for up to 24 months at -20°C.

• Vertical Electrophoresis system
• Power supplies
• Vortex or equivalent
• Microcentrifuge