BIO-HELIX - PM001-0500

BLUltra Prestained Protein Ladder(6.5 to 270 kDa)

Size: 500 μl



Note: Protein Marker / Protein Standard / SDS-PAGE / Western Blot / Protein Electrophoresis
Categories: Protein Marker/Ladder

The BLUltra Prestained Protein Ladder is a combination of 10 pre-stained proteins with molecular weights from 6.5 to 270 kDa. The 10 recombinant proteins are covalently coupled with blue chromophore, while 2 orange bands at 30 kDa and 270 kDa and a green band at 52 kDa serve as reference bands. The BLUltra Prestained Protein Ladder keeps track of the size and separation of proteins during SDS-polyacrylamide gel electrophoresis, approximating protein size and validating Western transfer efficiency on PVDF, nylon, or nitrocellulose membranes.

 

-Guide for Molecular Weight Estimation (kDa)

Migration patterns of BLUltra Prestained Protein Ladder in different electrophoresis conditions are listed below :

3 μl or 5 μl per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively. 2.5 μl per well for general Western transferring.

Note: The molecular weight of each protein (kDa) was measured against an unstained protein ladder in every electrophoresis condition. Additional data should be considered for a more accurate adjustment.

 

 

Nowadays, more and more researchers are studying the high molecular weight proteins, meaning they may need high-resolution prestained protein standards, especially with high molecular weights. That's why we're pleased to launch the color-enhanced BLUltra prestained protein standards.

As you may see the comparison data shown below, BLUltra shows a better resolution than the BLUeye & BlueRAY for the protein bands from 95 kDa to 270 kDa.

Lane 1: PMU12-0500 UNveil Unstained Protein Ladder (Bio-Helix) / 5μl
Lane 2: PM001-0500 BLUltra Prestained Protein Ladder (Bio-Helix) / 5μl
Lane 3: Bio-Rad 161-0374 Precision Plus Protein™ Dual Color Standards / 5μl
Lane 4: Thermo Scientific 26616 PageRuler Prestained Protein Ladder / 5μl
Lane 5: PM007-0500 BLUeye Prestained Protein Ladder (Bio-Helix) / 5μl
Lane 6: PMU12-0500 UNveil Unstained Protein Ladder (Bio-Helix) / 10μl
Lane 7: PM001-0500 BLUltra Prestained Protein Ladder (Bio-Helix) / 10μl
Lane 8: Bio-Rad 161-0374 Precision Plus Protein™ Dual Color Standards / 10μl
Lane 9: Thermo Scientific 26616 PageRuler Prestained Protein Ladder / 10μl
Lane 10: PM007-0500 BLUeye Prestained Protein Ladder (Bio-Helix) / 10μl

► One-Vial-Fits-All: New broad-range protein ladder from 6.5kDa to 270kDa.
► Higher Added Value: Tri-colors and 10 bands with premium quality.
► Better performance for higher molecular weight proteins.
► Clear separation map from 6.5 kDa to 270 kDa in the Tris-Glycine system.
► Enhanced transfer, wash, and stripping durabilities.

 

Storage Buffer : 
62.5 mM Tris-H3PO4 (pH 7.5 at 25 °C), 1 mM EDTA, 2% SDS, 10 mM DTT, 1 mM NaN3, 33% glycerol.

Quality Control : 
5 μl of BLUltra Prestained Protein Ladder (PM001-0500) resolves 10 bands in 4-20% SDS-PAGE (Tris-glycine buffer) and after Western blotting to the nitrocellulose membrane.

Storage: 
Stable for up to 2 weeks at 25°C.
Stable for up to 3 months at 4°C.
Stable for up to 24 months at -20°C.


 

Lane 1: PM019-0500 (Bio-Helix) 5ul
Lane 2: PM008-0500 (Bio-Helix) 5ul
Lane 3: PM007-0500 (Bio-Helix) 5ul
Lane 4: PM006-0500 (Bio-Helix) 5ul
Lane 5: PM001-0500 (Bio-Helix) 5ul
Lane 6: PM019-0500 (Bio-Helix) 5ul
Lane 7: PM001-0500 (Bio-Helix) 5ul
Lane 8: PMU12-0500 (Bio-Helix) 5ul
Lane 9: thermo Mark12 5ul
Lane 10: Bio-Rad 161-0317 5ul

*NuPAGE Bis-Tris 4-12% was used to run SDS-PAGE in MOPS and MES buffer. 5ul of protein ladder samples was loaded into each lane.

  • Vertical Electrophoresis system
  • Power supplies
  • Vortex or equivalent
  • Microcentrifuge

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