Qpd01 0100 panprobes universal mastermix

BIO-HELIX - QPD01-0100

PanProbes™ Universal qPCR MasterMix

Size: 100 reactions (20 μl vol)


Note: Probe-based qPCR Assays / TaqMan Assay / No ROX Reference Dye / No ROX Passive Fluorescent Dye / Gene expression analysis / qPCR Supermix / qPCR Premix
Categories: Real-Time PCR Reagent

* Enhanced Antibody-mediated hot-start DNA polymerase.
* Optimized for probe-based real-time PCR.
* Compatible with the majority of qPCR systems.

PanProbesTM Universal qPCR Master Mix is a 2x concentrated, ready-to-use master mix optimized for probe-based real-time PCR and compatible with the majority of commercially available real-time PCR systems (ROX-independent and ROX-dependent). It contains antibody-mediated hot-start Taq DNA
polymerase, dNTPs, MgCl2, enhancers, stabilizers and essentials for a success PCR reaction.         

Figure 1. Performance of PanProbes™ Universal qPCR MasterMix

The SARS-CoV-2 nucleocapsid (N1) gene with serial diluted DNA template concentrations are prepared and conducted by qPCR. Based on the detected N1 probe fluorescence values, the result shows the Ct values corresponding to the different concentration.

 

This Master Mix is compatible with the majority of commercially available real-time PCR systems.

  • ABI: 7500 Fast Series
  • Bio-Rad: CFX96
  • Roche: LightCycler Series
  • Agilent: Mx3005p
  • Qiagen: RotorGene 3000
  • Thermo Fisher: QuantStudio™ qPCR Systems

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